Dr. Jennifer Doudna: Genome Engineering with CRISPR-Cas9 In Breakthrough, Scientists Edit a Dangerous Mutation From Genes in Human Embryos Heritable Genome Editing
100
What is gel-electrophoresis?
Technology that was used to see the segmented DNA strands on agarose gel
100
What is federal law?
Reason why CRISPR-Cas9 cannot go into clinical trial
100
What is Preimplantation Genetic Diagnosis or PGD?
A technique that can be used in in vitro fertilization to identify embryos that are affected by a heritable genetic disease
100
What is biology, biotechnology, biomedicine?
Areas of sciences in which CRISPR-Cas9 technology can be applied to
100
What is breast and ovarian cancer, Huntington’s, Tay-Sachs, beta thalassemia, sickle cell anemia, cystic fibrosis, early-onset Alzheimer’s?
Possible conditions that may arise from specific inherited mutation
100
What is APOE4?
An allele that positively correlates with Alzheimer’s disease, but would probably not be selected for HGE because it offers some protection against liver damage by hepatitis C infection
100
What is ZFN and TALEN?
Two proteins that serve as protein-based recognition of DNA sequence
100
What is hypertrophic cardiomyopathy?
Disease that can cause sudden heart failure, often in young athletes
100
What is mitochondrial replacement?
Replacing mutated, disease causing mitochondria with normal mitochondria from a donor woman’s eggs
100
What is Cas9?
Protein that uses the sequence in guide RNA to create a double stranded DNA break?
100
What is disease-free and no transmission of diseases of descendants?
Some benefit of repairing mutations in embryos
100
What is mosaicism?
A technical issue with HGE, in which some cells in a resulting embryo do not contain a desired edit following HGE performed on a zygote
100
What is bacterial/archaeal chromosomes
Systems in which CRISPRs were first discovered?
100
What is eugenics?
Study to arrange reproduction to increase the occurrence of desirable heritable characteristics
100
What is heritable genome editing?
A form of germ line editing that includes the transfer of edited material for gestation with the intent to create a new human that is able to pass on this edit






CRISPR and Human Genome Editing

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