Stem Field Trip Jeoparady Jeopardy Template

Background	DNA Structure	Micropipetting	Gel Electropheresis	Molecular Modeling
100 Double Helix Model What did Watson, Crick, and Franklin discover?	100 Adenine, Guanine, Cytosine, and Thymine What are the nitrogenous bases of DNA?	100 They are used to measure very small volumes starting at 1 microliter What are micropipettes used for?	100 DNA What was put into the gel wells?	100 2 How many bonds can oxygen form?
200 Code for Genes What does DNA do?	200 3' to 5' In which direction is DNA read?	200 1000 microliters How many microliters are in 1mL?	200 Restriction Enzyme What can be used to cut DNA?	200 Deoxyribose sugar, Phosphate, and the nitrogenous base What are the three parts of a nucleotide?
300 DNa is double-stranded and RNA is single-stranded What is the difference between DNA and RNA?	300 Adenine - Thymine Guanine - Cytosine Which nitrogenous bases pair to each other?	300 After every use How often does the pipet tip need to be replaced?	300 The DNA ladder is a set of standards that are used to identify the approximate size of a molecule run on a gel. What is the DNA ladder used for?	300 Helix What is the structure of DNA?
400 Thymine is replaced with Uracil Which base pair(s) is different in RNA than in DNA?	400 Hydrogen bonding What is the intermolecular interaction that connects DNA strands?	400 Load the restriction enzymes and DNA into the gel What did we use the micropipettes for during the gel electrophoresis?	400 EcoR1 and Hind (III) What was one of the restriction enzyme used in today's lab?	400 Pyrimidines: Adenine and Guanine Purines: Cytosine and Thymine (Uracil in RNA) Which nitrogenous bases are the purines and which are the pyrimidines?
500 Using X-ray Chrystallography How did Watson, Crick, and Franklin discover the double helix model?	500 Pentose sugar What is the ring structure of the deoxyribose sugar?	500 P200 What micropipette was used during today's lab?	500 Exposing the stained gel to a UV light source causes the DNA/dye to fluoresce and become visible. Why does the gel need to be read under UV light?	500 The hydrogen attached at the carbon 2 position of the deoxyribose sugar is replaced with a hydroxyl group in the ribose sugar What is the difference between deoxyribose sugar and a ribose sugar?

STEM Field Trip Jeoparady

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