Techniques Jeopardy Template

Physical	Chemical	Immunological	Molecular	Genotypic/Phenotypic
100 What is the definition of physical methods The number of cells or quantity of byproducts	100 What is the definition of chemical methods Used to test for metabolic activity	100 What is the definition of immunological methods Used to test for growth response & cell measurements (DNA)	100 What are the basic steps in DNA probe hybridization isolate cells disrupt to obtain dsDNA convert to ssDNA add labeled probe hybridize	100 What is ribotyping distribution of restriction sites of ribosomal RNA
200 What are the 3 types of physical methods Microcalorimetry Flow Cytometry Impedence	200 What is ATP Measurement Rapid & specific method used to measure ATP (primary source of energy)	200 Radioactive label attached to an antigen & allowed to react with an antibody Radioimmunoassay	200 What is gene track assay combination of DNA hybridization & enzyme immunoassay; used to detect foodborne pathogens	200 What is PFGE pulse-field gel electrophoresis detect variations in nucleotide sequence in chromosomal DNA
300 Which technique determines the heat output and is used to estimate bacteria in ground meat Microcalorimetry	300 Radioactivity counter using 14C labelled metabolites Radiometry	300 What is ELISA used to detect specific antibodies & antigens	300 What are the steps in PCR denaturing, annealing & extension	300 What is the definition of genotypic genetic characteristics of an organism
400 Resistance in an electrical circuit Impedence	400 What are fluorogenic substrates used for plating media, MPN broths & membrane filter	400 Which type of ELISA consists of a secondary antibody binding to an enzyme Indirect	400 What are the types of PCR Real Time (quantify microorganisms) Reverse Transcriptase (turn RNA to DNA) Multiplex	400 What is the definition of phenotypic expressed characteristics of an organism
500 Determines properties of individual cells Flow Cytometry	500 What are the fluorogenic substrates MUG MUGal ONPG	500 What is fluorescent antibody & the two types Antibody made fluorescent by coupling it with a fluorescent compound; Direct (detects antigen) & Indirect (detects antibody)	500 What are the steps in gene track assay enrichment of food sample chemical lysis of cells hybridization immobilized hybrid with poly A dipstick detected by enzyme immunoassay	500 What are the types of phenotypic methods staining morphology serotyping antimicrobial resistance

Techniques

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