introduction | DNA damage | DNA damage and repair | random questions in this session. |
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What is DNA damage
This is a physical and chemical modification of DNA
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What is Inter-strand linkage
this is covalent bonding of nucleotides on a different strand
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What is DNA damage
this is detrimental to the system, must be eliminated
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What is Damage Recognition
this is recognition of damaged base by DNA glycosylase.
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What is the DNA damage causes
There are exogenous and endogenous
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What is the intrastrand linkage
this is covalent bonding of nucleotides within the same strand
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What is DNA repair
this is the process of cellular damage identification to sustain the integrity of the genome
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What is Site nicking or cutting
This AP endonuclease will recognize AP site and cut off the sit, creating nicked DNA strand
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What is Exogenous DNA damage
There are UV light, x-ray, and carcinogens
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What is base modifications
this is changing of nucleotides to become unnatural forms
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What is Cell cycle arrest
this copies all DNA material (damaged or not) and has to be done in order to prevent the passing of DNA damage
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What is polymerase and ligation
this DNA polymerase recognized nicked site and initiates repair, ended by nick sealing DNA ligase
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What is Endogenous DNA damage
There is ROS, base deamination, depurination
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What is Base pair mismatch
this is the replacement of A with G and C with T
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What is Apoptosis
Can happen due to 2 pathway: p53 dependent or independent
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What is Homologous recombination
this is a process where two homologous DNA exchange the DNA sequence
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What are the types of DNA damage
there are base modification, intrastrand linkage, interstrand linkage, and base-pair mismatch
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What is insertion and deletion
this is an extra nucleotide and deleted nucleotides
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What is G1/s
This is the primacy decision point known as the restriction point
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What is Check point activator
this is controlled by two master kinases, ATM and ATR
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